Difference between revisions of "Part:BBa K2332054:Design"
Paola handal (Talk | contribs) (→Design Notes) |
Paola handal (Talk | contribs) (→Source) |
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===Source=== | ===Source=== | ||
− | + | SpyCatcher sequence was obtained from [https://parts.igem.org/Part:BBa_K1159200 BBa_K1159200] and GFP from: | |
+ | [https://parts.igem.org/Part:BBa_E0040 BBa_E0040]. The DNA sequence was synthesised by Integrated DNA Technologies (IDT) | ||
===References=== | ===References=== |
Revision as of 23:47, 22 October 2017
GFP-SpyCatcher (constitutive)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 723
Design Notes
We removed the stop codon in GFP and placed the SpyCatcher sequence after a linker sequence and included a HisTag for protein purification.
Source
SpyCatcher sequence was obtained from BBa_K1159200 and GFP from: BBa_E0040. The DNA sequence was synthesised by Integrated DNA Technologies (IDT)