Difference between revisions of "Part:BBa K2332004:Design"

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===Design Notes===
 
===Design Notes===
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We had to reverse translate and codon optimize the protein sequence for its usage in E. coli and ensure no illegal sites were present.
 
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===Source===
 
===Source===

Revision as of 21:04, 22 October 2017


EL222, photosensitive DNA-binding protein


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 81
    Illegal AgeI site found at 306
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Source

Sequence obtained from the reverse translation of EL222 (UniProtKB/Swiss-Prot: [http://www.uniprot.org/uniprot/Q2NB98 Q2NB98.1]) then codon optimized for E. coli.

References