Difference between revisions of "Part:BBa K2486004"

 
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__NOTOC__
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<partinfo>BBa_K2486004 short</partinfo>
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The BBa_K486004 is a dtxR constitutive generator. This part is composed of:
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# A promoter ([https://parts.igem.org/wiki/index.php/Part:BBa_J23108 BBa_J23108]) from [https://parts.igem.org/Promoters/Catalog/Anderson Anderson promoter collection]
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# A ribosome binding site
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# A short spacer of 6bp (gtccta)
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# dtxR gene
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# A short spacer of 16bp (ccagctcagtccgcat)
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#
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REFERENCES:
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Boyd, J., Oza, M. N., & Murphy, J. R. (1990). Molecular cloning and DNA sequence analysis of a diphtheria tox iron-dependent regulatory element (dtxR) from Corynebacterium diphtheriae. Proceedings of the National Academy of Sciences, 87(15), 5968-5972.
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De Zoysa, A., Efstratiou, A., & Hawkey, P. M. (2005). Molecular characterization of diphtheria toxin repressor (dtxR) genes present in nontoxigenic Corynebacterium diphtheriae strains isolated in the United Kingdom. Journal of clinical microbiology, 43(1), 223-228.
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https://www.wikigenes.org/e/gene/e/2649688.html
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<!-- Add more about the biology of this part here
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===Usage and Biology===
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<!-- -->
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<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K2486005 SequenceAndFeatures</partinfo>
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<!-- Uncomment this to enable Functional Parameter display
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===Functional Parameters===
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<partinfo>BBa_K2486005 parameters</partinfo>
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Revision as of 04:18, 28 October 2017


dtxR generator

The BBa_K486004 is a dtxR constitutive generator. This part is composed of:

  1. A promoter (BBa_J23108) from Anderson promoter collection
  2. A ribosome binding site
  3. A short spacer of 6bp (gtccta)
  4. dtxR gene
  5. A short spacer of 16bp (ccagctcagtccgcat)


REFERENCES:

Boyd, J., Oza, M. N., & Murphy, J. R. (1990). Molecular cloning and DNA sequence analysis of a diphtheria tox iron-dependent regulatory element (dtxR) from Corynebacterium diphtheriae. Proceedings of the National Academy of Sciences, 87(15), 5968-5972.

De Zoysa, A., Efstratiou, A., & Hawkey, P. M. (2005). Molecular characterization of diphtheria toxin repressor (dtxR) genes present in nontoxigenic Corynebacterium diphtheriae strains isolated in the United Kingdom. Journal of clinical microbiology, 43(1), 223-228.

https://www.wikigenes.org/e/gene/e/2649688.html

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 509
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]