Difference between revisions of "Part:BBa K2429017:Design"

(→‎Design Notes)
(→‎Design Notes)
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===Design Notes===
 
===Design Notes===
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This part is human-codon optimized. We found a BsaI site in the middle of mKate Exon 2, so we use site-directed mutagenesis to change the base pair at position 1450 from a G to an A. We also mutated the HBG Intron 2 at position 654 from a C to an A because the paper we were referencing had mutated the HBG intron in the same way.
+
This part is human-codon optimized. We found a BsaI site in the middle of mKate Exon 2, so we use site-directed mutagenesis to change the base pair at position 1450 from a G to an A. We also mutated the HBG Intron 2 at position 888 from a C to an A because the paper we were referencing had mutated the HBG intron in the same way.
  
 
===Source===
 
===Source===

Revision as of 19:17, 22 October 2017


2 Exon mKate-HBG Reporter


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1582
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1582
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1582
    Illegal BamHI site found at 1
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1582
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1582
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1487
    Illegal SapI.rc site found at 55


Design Notes

This part is human-codon optimized. We found a BsaI site in the middle of mKate Exon 2, so we use site-directed mutagenesis to change the base pair at position 1450 from a G to an A. We also mutated the HBG Intron 2 at position 888 from a C to an A because the paper we were referencing had mutated the HBG intron in the same way.

Source

The source of this part

References