Difference between revisions of "Part:BBa K2294000"
Line 4: Line 4:
  
 
This part is a fusion of<br>
 
This part is a fusion of<br>
−
•  FLPe, a thermostable version of the <i>S. cerevisiae</i> FRT site specific recombinase FLP. <br>
+
•  FLPe, a thermostable version of the <i>S. cerevisiae</i> FRT site specific recombinase FLP and<br>
−
and<br>
+
 
•  NLS, a SV40 nuclear localization sequence. <br>
 
•  NLS, a SV40 nuclear localization sequence. <br>
  

Revision as of 14:10, 22 October 2017


FLPe + NLS

This part is a fusion of
• FLPe, a thermostable version of the S. cerevisiae FRT site specific recombinase FLP and
• NLS, a SV40 nuclear localization sequence.

See https://en.wikipedia.org/wiki/FLP-FRT_recombination and https://en.wikipedia.org/wiki/Nuclear_localization_sequence for more information.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 531
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]