Difference between revisions of "Chassis/Cell-Free Systems/Commercial E.coli T7 S30/Peak Time"
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| − | [[Image:T7_Peak_Time.JPG|thumb|800px|center|'''Graph 2. Graph of rate of GFP synthesis(mol per min) against time (minutes) at 4oC (blue), 25oC (red) and 37oC (green).''' The simple constitutive gene expression device [https://parts.igem.org/wiki/index.php/Part:BBa_E7104 BBa_E7104] has been used. Each of the three coloured lines show average measurements based on three replicates of the experiment. This graph is derived from Graph 1, with addition data from our GFP calibration curve. Please visit our OpenWetWare for the detailed protocol and raw data of the experiment.]] | + | [[Image:T7_Peak_Time.JPG|thumb|800px|center|'''Graph 2. Graph of rate of GFP synthesis (mol per min) against time (minutes) at 4oC (blue), 25oC (red) and 37oC (green).''' The simple constitutive gene expression device [https://parts.igem.org/wiki/index.php/Part:BBa_E7104 BBa_E7104] has been used. Each of the three coloured lines show average measurements based on three replicates of the experiment. This graph is derived from Graph 1, with addition data from our GFP calibration curve. Please visit our OpenWetWare for the detailed protocol and raw data of the experiment.]] |
Revision as of 13:44, 21 October 2007
Commercial E. coli T7 S30
| Cell-Free Systems | Chassis description | Optimum temperature | Peak time | Product stability | Expression capacity | Expression lifespan |
Peak Time
Graph 2. Graph of rate of GFP synthesis (mol per min) against time (minutes) at 4oC (blue), 25oC (red) and 37oC (green). The simple constitutive gene expression device BBa_E7104 has been used. Each of the three coloured lines show average measurements based on three replicates of the experiment. This graph is derived from Graph 1, with addition data from our GFP calibration curve. Please visit our OpenWetWare for the detailed protocol and raw data of the experiment.