Difference between revisions of "Part:BBa K2202002"
 
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<partinfo>BBa_K2202002 short</partinfo>
 
<partinfo>BBa_K2202002 short</partinfo>
  
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This part is an improvement to BBa_K2202003. This part works together with four other parts ( BBa_K2202001, BBa_K2202002, BBa_K2202003 and BBa_K2202004) and requires the chassis to contain HIV reverse transcriptase and Murine leukemia reverse transcriptase. The Part essentially produces a ncRNA which is later reverse transcribed to a single-stranded DNA that binds together with four other ssDNA produced to make a 2-dimensional  structure. This 2D structure, upon detecting a Huntington's Disease biomarker (hsa-miR-34b-3p), folds into a tetrahedron with a G-quadruplex. Colorimetric or fluorescent reagents such as ABTS and Thioflavin T can be used to detect this G-quadruplex. Hence our part functions as a diagnostic device for nucleic acid based biomarkers.  
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This part is an improvement to BBa_K2054003. This part works together with four other parts ( BBa_K2202001, BBa_K2202002, BBa_K2202003 and BBa_K2202004) and requires the chassis to contain HIV reverse transcriptase and Murine leukemia reverse transcriptase. The Part essentially produces a ncRNA which is later reverse transcribed to a single-stranded DNA that binds together with four other ssDNA produced to make a 2-dimensional  structure. This 2D structure, upon detecting a Huntington's Disease biomarker (hsa-miR-34b-3p), folds into a tetrahedron with a G-quadruplex. Colorimetric or fluorescent reagents such as ABTS and Thioflavin T can be used to detect this G-quadruplex. Hence our part functions as a diagnostic device for nucleic acid based biomarkers.  
  
  

Latest revision as of 12:06, 22 October 2017


ssDNA for producing in-vivo tetrahedral structure Strand3

This part is an improvement to BBa_K2054003. This part works together with four other parts ( BBa_K2202001, BBa_K2202002, BBa_K2202003 and BBa_K2202004) and requires the chassis to contain HIV reverse transcriptase and Murine leukemia reverse transcriptase. The Part essentially produces a ncRNA which is later reverse transcribed to a single-stranded DNA that binds together with four other ssDNA produced to make a 2-dimensional structure. This 2D structure, upon detecting a Huntington's Disease biomarker (hsa-miR-34b-3p), folds into a tetrahedron with a G-quadruplex. Colorimetric or fluorescent reagents such as ABTS and Thioflavin T can be used to detect this G-quadruplex. Hence our part functions as a diagnostic device for nucleic acid based biomarkers.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 101
    Illegal SapI site found at 272