Difference between revisions of "Part:BBa K2371000:Design"

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This biobrick is expected to generate the protein N-T7-dCas9 synthesized by part of split T7 polymerase and dCas9. The sequence of NT7 and dCas9 are linked via a linker. In consideration of the protein’s activity influenced by its function, the His-Tag must be linked to the C terminus of dCas9.
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__NOTOC__
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<partinfo>BBa_K2371000 short</partinfo>
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<partinfo>BBa_K2371000 SequenceAndFeatures</partinfo>
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===Design Notes===
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===Source===
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Sequence of T7 polymerase is obtained from Eco.li BL21(DE3), whose genome is genetically modified to contain the coding sequence for T7 polymerase.  
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===References===
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1.Yihao,Z.et al.,2017.Paired Design of dCas9 as a Systematic Platform for the Detection of Featured Nucleic Acid Sequences in Pathogenic Strains. ACS Synth. Biol., 2017, 6 (2), pp 211–216.
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2.Tiyun,H.et al.,2016.Engineered photoactivatable genetic switches based on the bacterium phage T7 RNA polymerase.ACS Synthetic Biology,http://pubs.acs.org on October 31, 2016.

Revision as of 13:18, 31 October 2017


N-t7-dCas9


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1973
    Illegal BamHI site found at 1708
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 2800
    Illegal NgoMIV site found at 3904
    Illegal NgoMIV site found at 3977
    Illegal NgoMIV site found at 4462
    Illegal NgoMIV site found at 5371
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Source

Sequence of T7 polymerase is obtained from Eco.li BL21(DE3), whose genome is genetically modified to contain the coding sequence for T7 polymerase.

References

1.Yihao,Z.et al.,2017.Paired Design of dCas9 as a Systematic Platform for the Detection of Featured Nucleic Acid Sequences in Pathogenic Strains. ACS Synth. Biol., 2017, 6 (2), pp 211–216. 2.Tiyun,H.et al.,2016.Engineered photoactivatable genetic switches based on the bacterium phage T7 RNA polymerase.ACS Synthetic Biology,http://pubs.acs.org on October 31, 2016.