Difference between revisions of "Part:BBa K2505000"
Line 1: Line 1:
−
 
 
__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K2505000 short</partinfo>
 
<partinfo>BBa_K2505000 short</partinfo>
−
 
−
<span style="margin-left: 25px;">This gene is derived from <i>A.thaliana</i> and optimized for <i>E.coli</i> codon in reference to the codon usage. AHK4 is a receptor of iP(isopentenyl adenine: kind of cytokinin). Once iP received by AHK4, AHK4 induces histidine kinase phosphorylation in <i>E.coli</i> cell. Then, arbitrary promoter is activated by the phosphorylation.
 
−
 
−
 
−
===Characterization===
 
−
 
−
<span style="margin-left: 25px;">We tested the function of AHK4 protein that receives iP and induces histidine kinase phosphorylation in <i>E.coli</i> cell.
 
−
 
−
Our objective was to characterize the function of AHK4 protein.
 
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here
−
===Usage and Biology===
 
  
 
<!-- -->
 
<!-- -->
Line 24: Line 13:
 
<partinfo>BBa_K2505000 parameters</partinfo>
 
<partinfo>BBa_K2505000 parameters</partinfo>
 
<!-- -->
 
<!-- -->
 +
 +
<span style="margin-left: 25px;">This gene is derived from <i>A.thaliana</i> and optimized for <i>E.coli</i> codon in reference to the codon usage. AHK4 is a receptor of iP(isopentenyl adenine: kind of cytokinin). Once iP received by AHK4, AHK4 induces histidine kinase phosphorylation in <i>E.coli</i> cell. Then, arbitrary promoter is activated by the phosphorylation.
 +
 +
 +
<html>
 +
</p>
 +
</html>
 +
__TOC__
 +
 +
==Characterization==
 +
 +
<span style="margin-left: 25px;">We tested the function of AHK4 protein that receives iP and induces histidine kinase phosphorylation in <i>E.coli</i> cell. Our objective was to characterize the function of AHK4 protein.
 +
===Materials & methods===
 +
 +
 +
 +
===Result===

Revision as of 07:07, 23 October 2017

rbs-ahk4

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 76
    Illegal BamHI site found at 1157
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 324
    Illegal SapI.rc site found at 1959


This gene is derived from A.thaliana and optimized for E.coli codon in reference to the codon usage. AHK4 is a receptor of iP(isopentenyl adenine: kind of cytokinin). Once iP received by AHK4, AHK4 induces histidine kinase phosphorylation in E.coli cell. Then, arbitrary promoter is activated by the phosphorylation.


Characterization

We tested the function of AHK4 protein that receives iP and induces histidine kinase phosphorylation in E.coli cell. Our objective was to characterize the function of AHK4 protein.

Materials & methods

===Result===