Difference between revisions of "Part:BBa K2325103"
Line 3: Line 3:
 
<partinfo>BBa_K2325103 short</partinfo>
 
<partinfo>BBa_K2325103 short</partinfo>
  
−
The BBa_K2325001 harbors a coding sequence of and formamidase(FOR) and phosphite dehydrogenase (PTDH) with a linker between them, which can simultaneously catalyzes the conversion of formamide to ammonia and phosphite to phosphate, respectively.
+
The BBa_K2325001 harbors a coding sequence of and formamidase(FOR) and phosphite dehydrogenase (PTDH) with a linker between them, which can simultaneously catalyzes the conversion of formamide to ammonia and phosphite to phosphate, respectively. The device also contains GST tag for increasing the solubility of exogenous protein.
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 09:01, 22 October 2017


An expression device for fusion protein of Formamidase and Phosphite dehydrogenase

The BBa_K2325001 harbors a coding sequence of and formamidase(FOR) and phosphite dehydrogenase (PTDH) with a linker between them, which can simultaneously catalyzes the conversion of formamide to ammonia and phosphite to phosphate, respectively. The device also contains GST tag for increasing the solubility of exogenous protein.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 821
    Illegal NgoMIV site found at 1308
    Illegal NgoMIV site found at 1503
    Illegal NgoMIV site found at 1650
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 159