Difference between revisions of "Part:BBa K2325001"
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Phosphite dehydrogenase is an enzyme involved in the production of phosphate for providing phosphorus source for the engineering bacteria. This part has been constructed into expression vectors to modify the metabolic pathway of phosphorus in E.coli. | Phosphite dehydrogenase is an enzyme involved in the production of phosphate for providing phosphorus source for the engineering bacteria. This part has been constructed into expression vectors to modify the metabolic pathway of phosphorus in E.coli. | ||
| − | == | + | <center><h4>KH<sub>2</sub><font color="green">PO<sub>3</sub></font> + H<sub>2</sub>O + NAD<sup>+</sup> ⇒ KH<sub>2</sub><font color="green">PO<sub>4</sub></font> + NADH</h4></center> |
| + | ===Gel analysis=== | ||
| + | [[File:BBa_K2325001--1.png]] | ||
| + | |||
| + | ===Enzymatic properities=== | ||
| + | <center> | ||
| + | [[File:BBa_K2325001--2.png|850px]]<br /> | ||
| + | <b>Figure 1.Enzymatic activity of phosphite dehydrogenase under different temperatures and pH</b> | ||
| + | </center> | ||
| + | <br /> | ||
| + | <center> | ||
| + | [[File:BBa_K2325001--3.png|750px]]<br /> | ||
| + | <b>Figure 2.The Michaelis constant of phosphite dehydrogenase</b> | ||
| + | </center> | ||
| + | <br /> | ||
| + | <center> | ||
| + | [[File:BBa_K2325001--4.png|500px]]<br /> | ||
| + | [[File:BBa K2325001--5.png]]<br /> | ||
| + | <b>Figure 3.Influence of metal ion on the enzymatic activity of phosphite dehydrogenase</b> | ||
| + | </center> | ||
| + | <br /> | ||
<!-- --> | <!-- --> | ||
<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> | ||
Revision as of 15:32, 27 October 2017
Phosphite dehydrogenase (PTDH)
The BBa_K2325001 harbors a coding sequence of Phosphite dehydrogenase (PTDH), which catalyzes the conversion of phosphite to phosphate.
Usage and Biology
Phosphite dehydrogenase is an enzyme involved in the production of phosphate for providing phosphorus source for the engineering bacteria. This part has been constructed into expression vectors to modify the metabolic pathway of phosphorus in E.coli.
KH2PO3 + H2O + NAD+ ⇒ KH2PO4 + NADH
Gel analysis
Enzymatic properities
Figure 1.Enzymatic activity of phosphite dehydrogenase under different temperatures and pH
Figure 2.The Michaelis constant of phosphite dehydrogenase
Figure 3.Influence of metal ion on the enzymatic activity of phosphite dehydrogenase
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]