Difference between revisions of "Part:BBa K2350021"
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===Promoter, RBS, and Terminator=== | ===Promoter, RBS, and Terminator=== | ||
+ | For more details of the connected promoter, RBS, and terminator, you can see in BBa_K2350018 for endolysin part, BBa_K2350019 for holin part, and BBa_2350004 for NrtA part. | ||
+ | |||
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here |
Revision as of 08:10, 22 October 2017
R0010-B0034-Holin-B0010-B0012-J23106-B0034-Endolysin-B0010-B0012-J23118-B0034-NrtA-B0015
Endolysin-Holin-NrtA
Promoter, RBS, and Terminator
For more details of the connected promoter, RBS, and terminator, you can see in BBa_K2350018 for endolysin part, BBa_K2350019 for holin part, and BBa_2350004 for NrtA part.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1035
Illegal NheI site found at 1058
Illegal NheI site found at 1757
Illegal NheI site found at 1780 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 2990
Illegal BamHI site found at 2360 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 1362
Illegal AgeI site found at 1432 - 1000COMPATIBLE WITH RFC[1000]