Difference between revisions of "Part:BBa K2505004"

Revision as of 09:08, 22 October 2017

atipt4-IVS-IRES-log1-polyA

This gene codes complete iP synthesis enzymes. This gene was introduced to human cell, EA.hy926 cell and produces iP(isopentenyl adenine: kind of cytokinin) as a signaling molecule. atipt4 gene and log1 gene is derived from A.thaliana, optimized for H.sapiens codon in reference to the codon usage. AtIPT4 is an enzyme(adenylate dimethylallyltransferase [EC:2.5.1.112]: cytokinin synthase) that necessary for a synthesis of iP. LOG1 is an enzyme(putative lysine decarboxylase family) that necessary for maturation of iP precursor.

Characterization

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 1193
Illegal XhoI site found at 2243
Illegal XhoI site found at 2255
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 739
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 881

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 <partinfo>BBa_K2505004 short</partinfo>
-<span style="margin-left: 25px;">This gene codes complete iP synthesis enzymes. This gene was introduced to human cell, EA.hy926 cell and produces iP(isopentenyl adenine: kind of cytokinin) as a signaling molecule. atipt4 gene and log1 gene is derived from <i>A.thaliana</i>, optimized for <i>H.sapiens</i> codon in reference to the codon usage. AtIPT4 is an enzyme(adenylate dimethylallyltransferase [EC:2.5.1.112]: cytokinin synthase) that necessary for a synthesis of iP. LOG1 is an enzyme(putative lysine decarboxylase family) that necessary for maturation of iP precursor.
+<span style="margin-left: 25px;">This gene codes complete iP synthesis enzymes. This gene was introduced to human cell, EA.hy926 cell and produces iP(isopentenyl adenine: kind of cytokinin) as a signaling molecule. atipt4 gene and log1 gene is derived from <i>A.thaliana</i>, optimized for <i>H.sapiens</i> codon in reference to the codon usage. AtIPT4 is an enzyme(adenylate dimethylallyltransferase '''[EC:2.5.1.112]''': cytokinin synthase) that necessary for a synthesis of iP. LOG1 is an enzyme(putative lysine decarboxylase family) that necessary for maturation of iP precursor.
 ===Characterization===