Difference between revisions of "Part:BBa K2505003"

Line 3: Line 3:
 
<partinfo>BBa_K2505003 short</partinfo>
 
<partinfo>BBa_K2505003 short</partinfo>
  
<span style="margin-left: 25px;">Log1 gene is derived from <i>A.thaliana</i> and optimized for <i>H.sapiens</i> codon in reference to codon usage. LOG1 is a enzyme(putative lysine decarboxylase family) that necessary for maturation of iP(isopentenyl adenine: kind of cytokinin).  
+
<span style="margin-left: 25px;">log1 gene is derived from <i>A.thaliana</i>, optimized for <i>H.sapiens</i> codon in reference to the codon usage. LOG1 is an enzyme(putative lysine decarboxylase family) that necessary for a maturation of iP precursor.
  
  
 
===Characterization===
 
===Characterization===
  
<span style="margin-left: 25px;">We tested the expression of LOG1 protein in human cell(EA.hy626) that necessary for maturation of iP(isopentenyl adenine: kind of cytokinin).  
+
<span style="margin-left: 25px;">We tested the expression of LOG1 protein in human cell(EA.hy626) that necessary for maturation of iP precursor.
  
 
  <!-- Add more about the biology of this part here
 
  <!-- Add more about the biology of this part here

Revision as of 07:31, 22 October 2017


IVS-IRES-log1

log1 gene is derived from A.thaliana, optimized for H.sapiens codon in reference to the codon usage. LOG1 is an enzyme(putative lysine decarboxylase family) that necessary for a maturation of iP precursor.


Characterization

We tested the expression of LOG1 protein in human cell(EA.hy626) that necessary for maturation of iP precursor.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 225
    Illegal XhoI site found at 1275
    Illegal XhoI site found at 1287
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]