Difference between revisions of "Part:BBa K2460009"
| Line 3: | Line 3: | ||
<partinfo>BBa_K2460009 short</partinfo> | <partinfo>BBa_K2460009 short</partinfo> | ||
| − | + | This is a recombination site attP for TG1 integrase ([[Part:BBa_K2460007|BBa_K2460007]]). When used with phiBT1 attB ([[Part:BBa_K2460008|BBa_K2460008]]) and TG1 integrase, it can flip a DNA sequence flanked by attB and attP sites, as well as integrating a circular DNA with a attB/P site into a linear/circular DNA with a attP/B site. | |
| + | For more information about kinetic characterization and the orthogonality between the integrase and some other serine recombinase, go to [[Part:BBa_K2460007|TG1 integrase]]. | ||
| + | |||
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
Revision as of 02:42, 2 November 2017
TG1 attP
This is a recombination site attP for TG1 integrase (BBa_K2460007). When used with phiBT1 attB (BBa_K2460008) and TG1 integrase, it can flip a DNA sequence flanked by attB and attP sites, as well as integrating a circular DNA with a attB/P site into a linear/circular DNA with a attP/B site. For more information about kinetic characterization and the orthogonality between the integrase and some other serine recombinase, go to TG1 integrase.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]