Difference between revisions of "Part:BBa K2450101"
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A non-self-cleaving TEV protease sequence with an N terminal mCherry tag and a C terminal His tag. The fluorophore tag allows for relative quantification of expression. The His tag allows for purification using a nickel column. | A non-self-cleaving TEV protease sequence with an N terminal mCherry tag and a C terminal His tag. The fluorophore tag allows for relative quantification of expression. The His tag allows for purification using a nickel column. | ||
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===Usage and Biology=== | ===Usage and Biology=== | ||
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This part does not require any other parts to be functional. | This part does not require any other parts to be functional. | ||
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Revision as of 15:47, 21 October 2017
TEV protease tagged with mCherry
A non-self-cleaving TEV protease sequence with an N terminal mCherry tag and a C terminal His tag. The fluorophore tag allows for relative quantification of expression. The His tag allows for purification using a nickel column.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 1444
- 1000COMPATIBLE WITH RFC[1000]
Usage and Biology
TEV protease is a well-characterised specific protease from the Tobacco Etch Virus, with a cleavage sequence of Glu-Asn-Leu-Tyr-Phe-Gln-(CUT)-Gly.
This is a non-self-cleaving protease as we wanted a constant level of TEV protease produced.
We have used TEV protease as both a substitute for cruzipain, another specific protease, and as a signal amplifier after simulated cruzipain cleavage.
This part does not require any other parts to be functional.