Difference between revisions of "Part:BBa K2350018:Design"
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===Design Notes=== | ===Design Notes=== | ||
　　Because the gene sequences of endolysin and holin in different species of bacteriophages are slightly different, we are not sure whether endolysin and holin from different species could cooperate well. Therefore, we choose the endolysin and the holin both are from enterobacteria phage T4. | 　　Because the gene sequences of endolysin and holin in different species of bacteriophages are slightly different, we are not sure whether endolysin and holin from different species could cooperate well. Therefore, we choose the endolysin and the holin both are from enterobacteria phage T4. | ||
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===Source=== | ===Source=== | ||
Revision as of 07:24, 22 October 2017
J23106-B0034-Endolysin-B0010-B0012
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 334
Illegal AgeI site found at 404 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
Because the gene sequences of endolysin and holin in different species of bacteriophages are slightly different, we are not sure whether endolysin and holin from different species could cooperate well. Therefore, we choose the endolysin and the holin both are from enterobacteria phage T4.
Source
The endolysin we used is from iGEM released parts, BBa_K112806, which is endolysin from enterobacteria phage T4.
References
- Mathias Schmelcher, David M Donovan, and Martin J Loessner. (2012). Bacteriophage endolysins as novel antimicrobials. Future Microbiol. 2012 Oct; 7(10): 1147–1171. doi: 10.2217/fmb.12.97
- 2014 Peking Team: http://2014.igem.org/Team:Peking/Suicide
- UniPort: http://www.uniprot.org/uniprot/P00720
- Wikipedia: https://en.wikipedia.org/wiki/Lysin