Difference between revisions of "Part:BBa K2374002:Design"
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===Design Notes=== | ===Design Notes=== | ||
− | site | + | We use PCR to clone the GAL80ts from the genomic DNA of "Saccharomyces cerevisiae"S288C. |
+ | |||
+ | Then to make 2 site-directed mutagenesis: | ||
+ | |||
EcoR I (325):GAATTC->GAGTTC | EcoR I (325):GAATTC->GAGTTC | ||
There are other mutations during the parts construction by accident: | There are other mutations during the parts construction by accident: |
Revision as of 03:46, 25 October 2017
GAL80ts (temperature dependent)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 993
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 19
Illegal BglII site found at 615 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 27
Illegal BsaI site found at 73
Design Notes
We use PCR to clone the GAL80ts from the genomic DNA of "Saccharomyces cerevisiae"S288C.
Then to make 2 site-directed mutagenesis:
EcoR I (325):GAATTC->GAGTTC There are other mutations during the parts construction by accident: 1142: A->G; aa: Lys->Arg 1154: A->G; aa: Glu->Gly
Source
Chromosome: XIII; NC_001145.3 (171594..172901) NOTE: Gal80ts is a mutation based on the gene above.