Difference between revisions of "Part:BBa K2374002"
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<partinfo>BBa_K2374002 short</partinfo> | <partinfo>BBa_K2374002 short</partinfo> | ||
| − | Gal80ts is a mutation based on Gal80 which is a transcription regulator in Saccharomyces cerevisiae S288C. | + | Gal80ts is a mutation based on Gal80 which is a transcription regulator in ''Saccharomyces cerevisiae'' S288C. |
The activity of Gal80ts is temperature dependent. At 25℃, it has a normal activity, which will bind to Gal4 and deactivate it. At 29℃, Gal80ts were inactivated and could not bind to Gal4. For the temperature between 25-29℃, we did not detect. | The activity of Gal80ts is temperature dependent. At 25℃, it has a normal activity, which will bind to Gal4 and deactivate it. At 29℃, Gal80ts were inactivated and could not bind to Gal4. For the temperature between 25-29℃, we did not detect. | ||
Revision as of 16:29, 25 October 2017
GAL80ts (temperature dependent)
Gal80ts is a mutation based on Gal80 which is a transcription regulator in Saccharomyces cerevisiae S288C. The activity of Gal80ts is temperature dependent. At 25℃, it has a normal activity, which will bind to Gal4 and deactivate it. At 29℃, Gal80ts were inactivated and could not bind to Gal4. For the temperature between 25-29℃, we did not detect.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 993
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 19
Illegal BglII site found at 615 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 27
Illegal BsaI site found at 73