Difference between revisions of "Part:BBa K2286003"
Line 3: | Line 3: | ||
<partinfo>BBa_K2286003 short</partinfo> | <partinfo>BBa_K2286003 short</partinfo> | ||
− | EchA is an epoxide hydrolase derived from Agrobacterium radiobacterAD1, which can undergo the ring-opening reaction of various epoxides. It is reported that EchA Shows better activity for Epoxohydrin, Epibromohydrin and Epifluorohydri in various epoxides[1]。 | + | EchA is an epoxide hydrolase derived from Agrobacterium radiobacterAD1, which can undergo the ring-opening reaction of various epoxides. |
− | Since this enzyme is derived from prokaryotic cells, the original sequence is suitable for expression in prokaryotic cells, and this sequence is codon optimized for petunia system and is more suitable for plant system expression. | + | It is reported that EchA Shows better activity for Epoxohydrin, Epibromohydrin and Epifluorohydri in various epoxides[1]。 |
+ | Since this enzyme is derived from prokaryotic cells, the original sequence is suitable for expression in prokaryotic cells, and this | ||
+ | sequence is codon optimized for petunia system and is more suitable for plant system expression. | ||
Revision as of 12:34, 20 October 2017
EchA: Epichlorohydrin epoxide hydrolase
EchA is an epoxide hydrolase derived from Agrobacterium radiobacterAD1, which can undergo the ring-opening reaction of various epoxides. It is reported that EchA Shows better activity for Epoxohydrin, Epibromohydrin and Epifluorohydri in various epoxides[1]。 Since this enzyme is derived from prokaryotic cells, the original sequence is suitable for expression in prokaryotic cells, and this sequence is codon optimized for petunia system and is more suitable for plant system expression.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]