Difference between revisions of "Part:BBa K2201243"

 
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Fusion protein of CFP and YFP with an amber codon in the linker
 
Fusion protein of CFP and YFP with an amber codon in the linker
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This part is an imroved version of the aaRS-test system “pFRY” (a Reporter Plasmid for Measuring ncAA Incorporation, BBa_K1416004) of the iGEM-Team Austin, Texas, 2014. We investigated that there were some issues with the choice of RFP and GFP for the system and decided to improve the part by using CFP and YFP, for they form a FRET system which leads to a more accurate distinction between the partial (CFP) and the whole (CFP-YFP) expressed fusion protein.
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<b>Important:</b> This part does not contain a promoter! For the functional part and the results, look up to BBa_K2201343.
  
 
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Revision as of 19:43, 24 October 2017


Fusion protein of CFP and YFP with an amber codon in the linker

Fusion protein of CFP and YFP with an amber codon in the linker

This part is an imroved version of the aaRS-test system “pFRY” (a Reporter Plasmid for Measuring ncAA Incorporation, BBa_K1416004) of the iGEM-Team Austin, Texas, 2014. We investigated that there were some issues with the choice of RFP and GFP for the system and decided to improve the part by using CFP and YFP, for they form a FRET system which leads to a more accurate distinction between the partial (CFP) and the whole (CFP-YFP) expressed fusion protein.

Important: This part does not contain a promoter! For the functional part and the results, look up to BBa_K2201343.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 718
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1308
    Illegal AgeI site found at 1420
  • 1000
    COMPATIBLE WITH RFC[1000]