Difference between revisions of "Part:BBa K2230005"
Line 5: Line 5:
 
PI promoter [BBa_K861170] is a modified version to Pcar with correction of -10 position in the promoter region, which gives the promoter stronger activity and a weaker CRP interaction. The PI promoter [BBa_K861170] was used as a major part in the work of team NCKU_Tainan in 2016. These two parts have been used in our work.  
 
PI promoter [BBa_K861170] is a modified version to Pcar with correction of -10 position in the promoter region, which gives the promoter stronger activity and a weaker CRP interaction. The PI promoter [BBa_K861170] was used as a major part in the work of team NCKU_Tainan in 2016. These two parts have been used in our work.  
  
−
Cloning: PI-RBS-RFP was amplified from RFP Coding Device (BBa_J04450) using a primer with PI-RBS (B0034) sequence. The PCR product was ligated to pSB1A3 cut by XbaI and PstI.  
+
===Cloning===
 +
PI-RBS-RFP was amplified from RFP Coding Device (BBa_J04450) using a primer with PI-RBS (B0034) sequence. The PCR product was ligated to pSB1A3 cut by XbaI and PstI.  
  
−
Function: RFP can be dose-dependently expressed in an increasing concentrations of glucose. According to teams WHU-China 2012 & NCKU_Tainan 2016, the promoter, PI, is glucose responsive.  
+
===Function===
 +
RFP may be dose-dependently expressed in an increasing concentrations of glucose. According to teams WHU-China 2012 & NCKU_Tainan 2016, the promoter, PI, is glucose responsive.  
 +
 
 +
 
 +
===Demonstration===
  
  

Revision as of 07:52, 25 October 2017


PI-RBS-RFP-T/pSB1A3

PI promoter [BBa_K861170] is a modified version to Pcar with correction of -10 position in the promoter region, which gives the promoter stronger activity and a weaker CRP interaction. The PI promoter [BBa_K861170] was used as a major part in the work of team NCKU_Tainan in 2016. These two parts have been used in our work.

Cloning

PI-RBS-RFP was amplified from RFP Coding Device (BBa_J04450) using a primer with PI-RBS (B0034) sequence. The PCR product was ligated to pSB1A3 cut by XbaI and PstI.

Function

RFP may be dose-dependently expressed in an increasing concentrations of glucose. According to teams WHU-China 2012 & NCKU_Tainan 2016, the promoter, PI, is glucose responsive.


Demonstration

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 617
    Illegal AgeI site found at 729
  • 1000
    COMPATIBLE WITH RFC[1000]