Difference between revisions of "Part:BBa K2302007"

 
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<partinfo>BBa_K2302007 short</partinfo>
 
<partinfo>BBa_K2302007 short</partinfo>
  
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it contains three enzymes of the serine synthesis pathway. Bacteria harboring this plasmidcan be induced by arabinose to synthesize and secrete serine. A SD sequence (RBS) were added to the front of each coding sequence of serA, serB and serC.
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This plasmid cords for lipase out the membrane,which can hydrolyze fat to fatty acids, it consists four basic parts: promoter T7, RBS, pelB and CALB. pelB is sequence cords for a functional signal peptide, which transport the fused protein CALB out the membrane efficiently, and CALB is a kind of lipase which can hydrolyze fat. By transforming this plasmid, a bacteria can secret lipase out the membrane,hydrolyzing fat in the environment to fatty acids.
  
 
<!-- Add more about the biology of this part here
 
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Revision as of 08:21, 22 October 2017


His+PelB+CALB

This plasmid cords for lipase out the membrane,which can hydrolyze fat to fatty acids, it consists four basic parts: promoter T7, RBS, pelB and CALB. pelB is sequence cords for a functional signal peptide, which transport the fused protein CALB out the membrane efficiently, and CALB is a kind of lipase which can hydrolyze fat. By transforming this plasmid, a bacteria can secret lipase out the membrane,hydrolyzing fat in the environment to fatty acids.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1050
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 52
    Illegal NgoMIV site found at 78
    Illegal NgoMIV site found at 771
    Illegal NgoMIV site found at 881
    Illegal AgeI site found at 573
    Illegal AgeI site found at 831
  • 1000
    COMPATIBLE WITH RFC[1000]