Difference between revisions of "Part:BBa K2332031:Design"
Paola handal (Talk | contribs) (→Design Notes) |
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===Design Notes=== | ===Design Notes=== | ||
− | + | We removed the stop codon in GFP and placed the SpyTag sequence after a linker sequence and included a HisTag for protein purification. | |
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===Source=== | ===Source=== |
Revision as of 22:07, 22 October 2017
GFP-SpyCatcher
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 662
Design Notes
We removed the stop codon in GFP and placed the SpyTag sequence after a linker sequence and included a HisTag for protein purification.
Source
Intimin protein sequence obtained from: http://www.uniprot.org/uniprot/P43261#sequences And reverse translated using: http://www.bioinformatics.org/sms2/rev_trans.html Intimin for the cell surface display of our tag was truncated according to Wentzel et al., 2001 SpyCatcher: BBa_K1159200 GFPmut 3b: Part:BBa_E0040