Difference between revisions of "Part:BBa K2206001"

Line 8: Line 8:
 
This part can be used to regulate the expression of any protein in response to the levels of hsa-miR-27b-3p present in a system.
 
This part can be used to regulate the expression of any protein in response to the levels of hsa-miR-27b-3p present in a system.
  
The transcripts produced by this part are toxic to E. coli. We recommend the use of a non-leaky promoter (for reference, we found BBa_K808000 was too leaky).
+
The transcripts produced by this part are toxic to E. coli. We recommend the use of an inducible non-leaky promoter to allow for amplification in E. coli. For reference, we found Bba_K808000 was too leaky.
  
 
This part contains a strong RBS sequence.
 
This part contains a strong RBS sequence.

Revision as of 14:14, 19 October 2017

Toehold switch for hsa-miR-27b-3p

Toehold switches are synthetic riboregulators that regulate gene expression post-transcriptionally. Gene expression can be activated in the presence of a cognate single stranded RNA molecule that contains an arbitrary sequence (the trigger RNA). The trigger RNA binds to the switch through base pairing, causing a conformational change that results in translation of the downstream protein coding region.

This part codes for a toehold switch that contains a region that is complementary to the microRNA hsa-miR-27b-3p (the trigger RNA). In the presence of hsa-miR-27b-3p, translation of the downstream protein coding region occurs.

This part can be used to regulate the expression of any protein in response to the levels of hsa-miR-27b-3p present in a system.

The transcripts produced by this part are toxic to E. coli. We recommend the use of an inducible non-leaky promoter to allow for amplification in E. coli. For reference, we found Bba_K808000 was too leaky.

This part contains a strong RBS sequence.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]