Difference between revisions of "Part:BBa K2206000"
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This part can be used to regulate the expression of any protein in response to the levels of hsa-miR-15b-5p present in a system. | This part can be used to regulate the expression of any protein in response to the levels of hsa-miR-15b-5p present in a system. | ||
− | The transcripts produced by this part are toxic to E. coli. We recommend the use of a non-leaky promoter (for reference, we found | + | The transcripts produced by this part are toxic to E. coli. We recommend the use of a non-leaky promoter (for reference, we found BBa_K808000 was too leaky). |
This part contains a strong RBS sequence. | This part contains a strong RBS sequence. |
Revision as of 13:41, 19 October 2017
Toehold switch for hsa-miR-15b-5p
Toehold switches are synthetic riboregulators that regulate gene expression post-transcriptionally. Gene expression can be activated in the presence of a cognate single stranded RNA molecule that contains an arbitrary sequence (the trigger RNA). The trigger RNA binds to the switch through base pairing, causing a conformational change that results in translation of the downstream protein coding region.
This part codes for a toehold switch that contains a region that is complementary to the microRNA hsa-miR-15b-5p (the trigger RNA). In the presence of hsa-miR-15b-5p, translation of the downstream protein coding region occurs.
This part can be used to regulate the expression of any protein in response to the levels of hsa-miR-15b-5p present in a system.
The transcripts produced by this part are toxic to E. coli. We recommend the use of a non-leaky promoter (for reference, we found BBa_K808000 was too leaky).
This part contains a strong RBS sequence.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]