Difference between revisions of "Part:BBa K2387059:Design"
(→Design Notes) |
|||
| Line 7: | Line 7: | ||
===Design Notes=== | ===Design Notes=== | ||
| − | + | Removed illegal PstI restriction site by silent mutation (GCA GAA --> GCC GAA) at Ala356 | |
| − | + | ||
| − | + | ||
===Source=== | ===Source=== | ||
Latest revision as of 13:58, 19 October 2017
Extracellular domain of ISG65 of T.b. gambiense
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 751
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Removed illegal PstI restriction site by silent mutation (GCA GAA --> GCC GAA) at Ala356
Source
PCR amplified from genomic DNA of Trypanosoma brucei gambiense