Difference between revisions of "Part:BBa K2387058:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | + | Removed illegal SpeI restriction site by silent mutation (ACT AGT --> ACA AGT) at Thr336 | |
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===Source=== | ===Source=== | ||
Latest revision as of 13:54, 19 October 2017
Extracellular domain of ISG64 of T.b. gambiense
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 739
Design Notes
Removed illegal SpeI restriction site by silent mutation (ACT AGT --> ACA AGT) at Thr336
Source
PCR amplified from genomic DNA of Trypanosoma brucei gambiense