Difference between revisions of "Part:BBa K2443031"
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<partinfo>BBa_K2443031 short</partinfo> | <partinfo>BBa_K2443031 short</partinfo> | ||
− | Release factor 3 (RF3) allows for the termination of translation through the recognition of the stop codon in an mRNA sequence. RF3 has been Codon optimized for use in <i>Escherichia coli</i> and contains a N-Terminal | + | Release factor 3 (RF3) allows for the termination of translation through the recognition of the stop codon in an mRNA sequence. RF3 has been Codon optimized for use in <i>Escherichia coli</i> and contains a N-Terminal hexahistidine tag. Under the regulation of T7 Promoter, RBS and double terminator. |
Revision as of 00:07, 1 November 2017
RF3 optimized for expression in E. coli
Release factor 3 (RF3) allows for the termination of translation through the recognition of the stop codon in an mRNA sequence. RF3 has been Codon optimized for use in Escherichia coli and contains a N-Terminal hexahistidine tag. Under the regulation of T7 Promoter, RBS and double terminator.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 919
Illegal AgeI site found at 980 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1143