Difference between revisions of "Part:BBa F2620"
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|-
 
|-
 
|rowspan="3"|[[Part:BBa_F2620:Transfer Function|'''Transfer Function''']]
 
|rowspan="3"|[[Part:BBa_F2620:Transfer Function|'''Transfer Function''']]
−
|[[Input Swing|''Input Swing'']]
+
|''Maximum Output''
−
|1E-9 - 1E-6 M [[3OC6HSL|3OC<sub>6</sub>HSL]], exogenous
+
|6.7 [[PoPS]] cell<sup>-1</sup>
 
|-
 
|-
−
|[[Output Swing|''Output Swing'']]
+
|''Hill coefficient''
−
|0±1 - 503±1 GFP molecules cfu<sup>-1</sup> s<sup>-1</sup>
+
|1.2
 
|-
 
|-
 
|[[Switch Point|''Switch Point'']]
 
|[[Switch Point|''Switch Point'']]
−
|7E-9 ±1E-9 M [[3OC6HSL|3OC<sub>6</sub>HSL]], exogenous
+
|2E-9 M [[3OC6HSL|3OC<sub>6</sub>HSL]], exogenous
 
|-
 
|-
−
|rowspan="4"|[[Part:BBa_F2620:Response time|'''Response time:''']]
+
|[[Part:BBa_F2620:Response time|'''Response time:''']]
−
|''Time from 0 - 50% output''
+
|<1 min
−
|9 min
+
 
|-
 
|-
−
|''Time from 0 - 90% output''
+
|rowspan="2"|[[Part:BBa_F2620:Specificity|'''Input compatibility''']]
−
|27 min
+
−
|-
+
−
|''Time from 100 - 50% output''
+
−
|# min
+
−
|-
+
−
|''Time from 100 - 5% output''
+
−
|# min
+
−
|-
+
−
|rowspan="2"|[[Part:BBa_F2620:Specificity|'''Specificity''']]
+
 
|''Strong response to''
 
|''Strong response to''
 
|[[3OC6HSL|3OC<sub>6</sub>HSL]], C<sub>6</sub>HSL , C<sub>7</sub>HSL, 3OC<sub>8</sub>HSL, C<sub>8</sub>HSL
 
|[[3OC6HSL|3OC<sub>6</sub>HSL]], C<sub>6</sub>HSL , C<sub>7</sub>HSL, 3OC<sub>8</sub>HSL, C<sub>8</sub>HSL
Line 46: Line 36:
 
|rowspan="2"|[[Part:BBa_F2620:Stability|'''Stability''']]
 
|rowspan="2"|[[Part:BBa_F2620:Stability|'''Stability''']]
 
|[[Genetic Stability|''Genetic Stability'']]<br>(Low/High Input)
 
|[[Genetic Stability|''Genetic Stability'']]<br>(Low/High Input)
−
|>92/74 generations  
+
|>92/<74 generations  
 
|-
 
|-
 
|[[Performance Stability|''Performance Stability'']]<br>(Low/High Input)
 
|[[Performance Stability|''Performance Stability'']]<br>(Low/High Input)
−
|>92/74 generations
+
|>92/<74 generations
−
|-
+
−
|rowspan="5"|Demand
+
−
|rowspan="2"|[[Translation Demand|''Translation Demand:'']]<br>(Low/High Input)
+
−
|5040/141600 charged tRNA cfu<sup>-1</sup> s<sup>-1</sup>
+
 
|-
 
|-
−
|336/9440 ribosomes sequestered cfu<sup>-1</sup>
+
|rowspan="4"|Demand
 +
|rowspan="1"|Internal Demand<br>(Low/High Input)
 +
|Not measured
 
|-
 
|-
−
|rowspan="2"|[[Transcription Demand|''Transcription Demand:'']]<br>(Low/High Input)
+
|rowspan="2"|[[Transcription Demand|''Transcription output demand:'']]<br>(Low/High Input)
−
|(#/#) nucleotides cfu<sup>-1</sup> s<sup>-1</sup>
+
|(nd/6xNt) nucleotides cell<sup>-1</sup> s<sup>-1</sup>
 
|-
 
|-
−
|(#/#) RNAP sequestered cfu<sup>-1</sup>
+
|(nd/1.5E-1xNt) RNAP cell<sup>-1</sup>
 
|-
 
|-
 
|[[Growth Rate|''Growth Rate'']]<br>(Low/High Input)
 
|[[Growth Rate|''Growth Rate'']]<br>(Low/High Input)
−
|54/59 min doubling time
+
|54/59 min Doubling time
 
|}
 
|}
 
</center>
 
</center>

Revision as of 01:50, 20 October 2007

Input: 3OC6HSL
Output: PoPS

tetR
R0040		LuxR
I0462		lux pR
R0062
 BBa F2620Icon.png

Part Main Page        Transfer Function        Specificity        Response time        Stability        Add Data       


Description
A transcription factor [LuxR] (BBa_C0062) that is active in the presence of cell-cell signaling molecule 3OC6HSL is controlled by a tetR regulatable operator (BBa_R0040). Device input is 3OC6HSL. Device output is PoPS from a LuxR-regulated operator. If used in a cell containing TetR then a second input signal such as [http://openwetware.org/wiki/ATc aTc] can be used to produce a logical AND function. Get the pdf datasheet here.


Performance

Experiment1 Characteristic1 Value1
Transfer Function Maximum Output 6.7 PoPS cell-1
Hill coefficient 1.2
Switch Point 2E-9 M 3OC6HSL, exogenous
Response time: <1 min
Input compatibility Strong response to 3OC6HSL, C6HSL , C7HSL, 3OC8HSL, C8HSL
Weak response to C4HSL, C10HSL, C12HSL
Stability Genetic Stability
(Low/High Input) 		>92/<74 generations
Performance Stability
(Low/High Input) 		>92/<74 generations
Demand Internal Demand
(Low/High Input) 		Not measured
Transcription output demand:
(Low/High Input) 		(nd/6xNt) nucleotides cell-1 s-1
(nd/1.5E-1xNt) RNAP cell-1
Growth Rate
(Low/High Input) 		54/59 min Doubling time

1Measured by Ania Labno and Barry Canton 2006-2007

Compatibility
Chassis: Device has been shown to work in BBa_V1000,BBa_V1001,BBa_V1002
Plasmids: Device has been shown to work on pSB3K3 and pSB1A3
Devices: Device has been shown to work with BBa_E0430, BBa_E0434, BBa_E0240
Crosstalk with systems containing BBa_C0040.
Cell-Cell Signaling Systems: Crosstalk with devices using 3OC6HSL, C6HSL, C7HSL, C8HSL, C10HSL.