Difference between revisions of "Part:BBa K2443030"

Latest revision as of 00:06, 1 November 2017

RF2 optimized for expression in E. coli

Release factor 2 (RF2) allows for the termination of translation through the recognition of the stop codon in an mRNA sequence. RF2 has been Codon optimized for use in Escherichia coli and contains a N-Terminal hexahistidine tag. Under the regulation of T7 Promoter, RBS and double terminator.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 636
Illegal BglII site found at 1093
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

Revision as of 00:48, 19 October 2017 (view source) Registry (Talk \| contribs)		Latest revision as of 00:06, 1 November 2017 (view source) Harlando (Talk \| contribs)
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	<partinfo>BBa_K2443030 short</partinfo>		<partinfo>BBa_K2443030 short</partinfo>

−	Release factor 2 (RF2) allows for the termination of translation through the recognition of the stop codon in an mRNA sequence. RF2 has been Codon optimized for use in <i>Escherichia coli</i> and contains a N-Terminal ~~histidine~~ tag. Under the regulation of T7 Promoter, RBS and double terminator.	+	Release factor 2 (RF2) allows for the termination of translation through the recognition of the stop codon in an mRNA sequence. RF2 has been Codon optimized for use in <i>Escherichia coli</i> and contains a N-Terminal hexahistidine tag. Under the regulation of T7 Promoter, RBS and double terminator.