Difference between revisions of "Part:BBa K2259010"
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[[Image:Rop protein 3d small.gif|right|500px|frame|<b>Formatting test</b> Figure 1:  Plasmid map of the plasmid p68 used for the characterization of P''alcA''.]]
 
[[Image:Rop protein 3d small.gif|right|500px|frame|<b>Formatting test</b> Figure 1:  Plasmid map of the plasmid p68 used for the characterization of P''alcA''.]]
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===Biology===
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===Introduction===
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Repressor of primer (ROP) is a small dimeric protein that participates in ColE1 plasmid family copy number contro, by increasing affinity between two complementary RNAs - RNA I (Replication inhibitor) and RNA II (Replication activator). <ref>Castagnoli L, Scarpa M, Kokkinidis M, Banner DW, Tsernoglou D, Cesareni G. Genetic and structural analysis of the ColE1 Rop (Rom) protein. The EMBO Journal. 1989;8(2):621-629.</ref>
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==Biology==
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Repressor of primer (ROP) is a small dimeric protein that participates in ColE1 plasmid family copy number contro, by increasing affinity between two complementary RNAs - RNA I (Replication inhibitor) and RNA II (Replication activator). <ref>Castagnoli L, Scarpa M, Kokkinidis M, Banner DW, Tsernoglou D, Cesareni G. Genetic and structural analysis of the ColE1 Rop (Rom) protein. The EMBO Journal. 1989;8(2):621-629.</ref> By increasing affinity of the two RNA molecules Rop decreases the rate of of plasmid replication initiation events.
  
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Rop dimer is a bundle of four tightly packed alpha helices that are held by hydrophobic interactions.
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Rop dimer is a bundle of four tightly packed alpha helices that are held by hydrophobic interactions (Fig. 1).
  
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===Usage in SynORI system===
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==Usage in SynORI system==
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===Characterization of Rop protein (Vilnius-Lithuania 2017)===
  
  
 
==References==
 
==References==
 
<references />
 
<references />

Revision as of 23:21, 18 October 2017


Rop protein - global copy number inhibitor (SynORI framework)

Rop (also known as repressor of primer) is a small protein responsible for keeping the copy number of ColE1 and related bacterial plasmids low in E. coli. Inhibits plasmid replication of all SynORI framework plasmid groups non-specifically, also other ColE1 replicon plasmids.



Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Formatting test Figure 1: Plasmid map of the plasmid p68 used for the characterization of PalcA.

Introduction

Biology

Repressor of primer (ROP) is a small dimeric protein that participates in ColE1 plasmid family copy number contro, by increasing affinity between two complementary RNAs - RNA I (Replication inhibitor) and RNA II (Replication activator). [1] By increasing affinity of the two RNA molecules Rop decreases the rate of of plasmid replication initiation events.

Rop dimer is a bundle of four tightly packed alpha helices that are held by hydrophobic interactions (Fig. 1).

Usage in SynORI system

Characterization of Rop protein (Vilnius-Lithuania 2017)

References

  1. ↑ Castagnoli L, Scarpa M, Kokkinidis M, Banner DW, Tsernoglou D, Cesareni G. Genetic and structural analysis of the ColE1 Rop (Rom) protein. The EMBO Journal. 1989;8(2):621-629.