Difference between revisions of "Part:BBa K2259010"

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===Usage in SynORI system===
 
===Usage in SynORI system===
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==References==
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Revision as of 23:15, 18 October 2017


Rop protein - global copy number inhibitor (SynORI framework)

Rop (also known as repressor of primer) is a small protein responsible for keeping the copy number of ColE1 and related bacterial plasmids low in E. coli. Inhibits plasmid replication of all SynORI framework plasmid groups non-specifically, also other ColE1 replicon plasmids.



Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Formatting test Figure 1: Plasmid map of the plasmid p68 used for the characterization of PalcA.

Biology

Repressor of primer (ROP) is a small dimeric protein that participates in ColE1 plasmid family copy number contro, by increasing affinity between two complementary RNAs - RNA I (Replication inhibitor) and RNA II (Replication activator). [1]

Rop dimer is a bundle of four tightly packed alpha helices that are held by hydrophobic interactions.

Usage in SynORI system

References

  1. Castagnoli L, Scarpa M, Kokkinidis M, Banner DW, Tsernoglou D, Cesareni G. Genetic and structural analysis of the ColE1 Rop (Rom) protein. The EMBO Journal. 1989;8(2):621-629.