Difference between revisions of "Part:BBa K2332020:Design"
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===Design Notes=== | ===Design Notes=== | ||
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===Source=== | ===Source=== |
Revision as of 23:11, 22 October 2017
LuxCDABE (E. coli bioluminescence)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 2562
Illegal AgeI site found at 2829
Illegal AgeI site found at 3357
Illegal AgeI site found at 3938
Illegal AgeI site found at 4385
Illegal AgeI site found at 5263
Illegal AgeI site found at 5478 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
Source
Vibrio fischeri LuxR and Lux operon GenBank: AF170104.1 from which LuxC, D, A, B, E genes were identified and codon optimized for E. coli. The 5kb construct was split into 8 gblock fragments (700-900bp each) and ordered from IDT