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===Biology===
 
===Biology===
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[[Image:https://static.igem.org/mediawiki/parts/thumb/7/7f/Rop_protein_3d_structure.gif/533px-Rop_protein_3d_structure.gif|left|500px|frame|<b>Figure 1.</b> 3D structure of ROP protein.]]
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[[Image:https://parts.igem.org/File:Rop_protein_3d_structure.gif|left|500px|frame|<b>Figure 1.</b> 3D structure of ROP protein.]]
 
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Revision as of 22:21, 18 October 2017


Rop protein - global copy number inhibitor (SynORI framework)

Rop (also known as repressor of primer) is a small protein responsible for keeping the copy number of ColE1 and related bacterial plasmids low in E. coli. Inhibits plasmid replication of all SynORI framework plasmid groups non-specifically, also other ColE1 replicon plasmids.



Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Biology

File:Https://parts.igem.org/File:Rop protein 3d structure.gif
Figure 1. 3D structure of ROP protein.

Test Test Test Test Test Test Test Test Test Test Test Test Test Test Test Test Test Test Test Test Test Test Test Test Test Test Test Test Test Test Test Test Test Test Test Test Test Test Test Test Test Test Test Test Test Test Test Test Test Test Test Test Test Test Test Test Test Test Test Test Test Test Test Test Test Test Test Test Test Test Test Test Test Test Test Test Test Test Test Test Test Test Test Test Test Test Test Test Test Test Test Test Test Test Test Test Test Test Test Test Test Test Test Test Test Test Test Test Test Test Test Test Test Test Test Test Test Test Test Test Test Test Test Test Test Test Test Test Test Test Test Test Test Test Test Test Test Test Test Test Test Test Test Test Test Test Test Test Test Test Test Test Test Test Test Test Test Test Test Test Test Test Test Test Test Test Test Test Test Test Test Test Test Test Test Test Test Test Test Test Test Test Test Test Test Test Test Test Test Test Test Test Test Test Test Test Test Test Test Test Test Test Test Test Test Test Test Test Test Test Test Test Test Test Test Test Test Test Test Test Test Test Test Test Test Test Test Test Test Test Test Test Test Test Test Test Test Test Test Test Test Test Test Test Test Test Test Test Test Test Test Test Test Test Test Test Test Test Test Test Test Test Test Test Test Test Test Test Test Test Test Test Test Test Test Test Test Test Test Test Test Test Test Test Test Test Test Test Test Test Test Test

Formatting test Figure 1: Plasmid map of the plasmid p68 used for the characterization of PalcA.

Usage in SynORI system