Difference between revisions of "Part:BBa K2443015"

Latest revision as of 23:50, 31 October 2017

PheRS Beta subunit optimized for expression in E. coli

Phenylalanine tRNA synthetase responsible for attaching phenylalanine onto its tRNA to form an aminoacyl-tRNA. Codon optimized for use in Escherichia coli. Contains a C-terminal hexahistidine tag with a serine glycine linker. Under the regulation of T7 Promoter, RBS and double terminator.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 1906
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal SapI site found at 1976

Revision as of 16:05, 18 October 2017 (view source) Registry (Talk \| contribs)		Latest revision as of 23:50, 31 October 2017 (view source) Harlando (Talk \| contribs)
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	<partinfo>BBa_K2443015 short</partinfo>		<partinfo>BBa_K2443015 short</partinfo>

−	Phenylalanine tRNA synthetase responsible for attaching phenylalanine onto its tRNA to form an aminoacyl-tRNA. Codon optimized for use in <i>Escherichia coli</i>. Contains a C-terminal ~~histidine~~ tag with a serine glycine linker. Under the regulation of T7 Promoter, RBS and double terminator.	+	Phenylalanine tRNA synthetase responsible for attaching phenylalanine onto its tRNA to form an aminoacyl-tRNA. Codon optimized for use in <i>Escherichia coli</i>. Contains a C-terminal hexahistidine tag with a serine glycine linker. Under the regulation of T7 Promoter, RBS and double terminator.