Difference between revisions of "Part:BBa K2443006"

Latest revision as of 23:22, 31 October 2017

GluRS optimized for expression in E. coli

Glutamic acid tRNA synthetase responsible for attaching glutamic acid onto its tRNA to form an aminoacyl-tRNA. Codon optimized for use in Escherichia coli. Contains a N-terminal hexahistidine tag with a serine glycine linker. Under the regulation of T7 Promoter, RBS and double terminator.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 751
1000
INCOMPATIBLE WITH RFC[1000]
Illegal SapI.rc site found at 422

Revision as of 13:58, 18 October 2017 (view source) Registry (Talk \| contribs)		Latest revision as of 23:22, 31 October 2017 (view source) Harlando (Talk \| contribs)
Line 3:		Line 3:
	<partinfo>BBa_K2443006 short</partinfo>		<partinfo>BBa_K2443006 short</partinfo>

−	Glutamic acid tRNA synthetase responsible for attaching glutamic acid onto its tRNA to form an aminoacyl-tRNA. Codon optimized for use in <i>Escherichia coli</i>. Contains a N-terminal ~~histidine~~ tag with a serine glycine linker. Under the regulation of T7 Promoter, RBS and double terminator.	+	Glutamic acid tRNA synthetase responsible for attaching glutamic acid onto its tRNA to form an aminoacyl-tRNA. Codon optimized for use in <i>Escherichia coli</i>. Contains a N-terminal hexahistidine tag with a serine glycine linker. Under the regulation of T7 Promoter, RBS and double terminator.