Difference between revisions of "Part:BBa I742114"
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<partinfo>BBa_I742114 short</partinfo> | <partinfo>BBa_I742114 short</partinfo> | ||
| − | gene is from Pseudomonas fluorescens | + | The gene is from ''Pseudomonas fluorescens''. The sequence was submitted to GeneArt for synthesis including codon optimisation (''Pseudomonas'' has a very high GC content whereas ''E. coli'' and ''Lactobacillus'' have much lower GC content). |
| − | encodes feruoyl CoA synthase (ligates acetyl-CoA onto ferulic acid) | + | *encodes feruoyl CoA synthase (ligates acetyl-CoA onto ferulic acid) |
| − | produces feruloyl CoA from ferrulic acid | + | *produces feruloyl CoA from ferrulic acid |
| − | catalyses the penultimate of the vanillin biosynthesis pathway | + | *catalyses the penultimate step of the vanillin biosynthesis pathway |
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Revision as of 17:59, 26 October 2007
Feruloyl-CoA synthase (fcs)
The gene is from Pseudomonas fluorescens. The sequence was submitted to GeneArt for synthesis including codon optimisation (Pseudomonas has a very high GC content whereas E. coli and Lactobacillus have much lower GC content).
- encodes feruoyl CoA synthase (ligates acetyl-CoA onto ferulic acid)
- produces feruloyl CoA from ferrulic acid
- catalyses the penultimate step of the vanillin biosynthesis pathway
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 30
Illegal NgoMIV site found at 384
Illegal NgoMIV site found at 1107
Illegal AgeI site found at 535
Illegal AgeI site found at 1072
Illegal AgeI site found at 1533 - 1000COMPATIBLE WITH RFC[1000]