Difference between revisions of "Part:BBa I742112"
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<partinfo>BBa_I742112 short</partinfo> | <partinfo>BBa_I742112 short</partinfo> | ||
| − | enoyl CoA hydratase (ech) was | + | The sequence of enoyl CoA hydratase (ech) was derived from the ''Pseudomonas fluorescens'' genome. The sequence was submitted to GeneArt for synthesis including codon optimisation (''Pseudomonas'' has a very high GC content whereas ''E. coli'' and ''Lactobacillus'' have much lower GC content). |
Ech converts feruloyl CoA to vanillin (fig. 3) and catalyses the final stage of the vanillin biosynthesis pathway. | Ech converts feruloyl CoA to vanillin (fig. 3) and catalyses the final stage of the vanillin biosynthesis pathway. | ||
| − | Involved in the synthesis of 'natural' vanilla flavouring | + | Involved in the synthesis of 'natural' vanilla flavouring. |
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Latest revision as of 17:57, 26 October 2007
enoyl CoA hydratase (ech) gene
The sequence of enoyl CoA hydratase (ech) was derived from the Pseudomonas fluorescens genome. The sequence was submitted to GeneArt for synthesis including codon optimisation (Pseudomonas has a very high GC content whereas E. coli and Lactobacillus have much lower GC content).
Ech converts feruloyl CoA to vanillin (fig. 3) and catalyses the final stage of the vanillin biosynthesis pathway.
Involved in the synthesis of 'natural' vanilla flavouring.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 150
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 178
- 1000COMPATIBLE WITH RFC[1000]