Difference between revisions of "Part:BBa K2407102:Design"
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===Design Notes=== | ===Design Notes=== | ||
This sequence is modified for use in yeast or other AT-rich fungi. | This sequence is modified for use in yeast or other AT-rich fungi. | ||
+ | This part expresses the vika gene which will react with vox site in the mating so as to trigger Vika-Vox system. | ||
Revision as of 15:52, 21 October 2017
control the expression of vika gene
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1482
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
This sequence is modified for use in yeast or other AT-rich fungi. This part expresses the vika gene which will react with vox site in the mating so as to trigger Vika-Vox system.
Source
We use overlap PCR to connect the segments(TDH3p,vika and TDH2t).