Difference between revisions of "Part:BBa K2232003"

 
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<partinfo>BBa_K2232003 short</partinfo>
 
<partinfo>BBa_K2232003 short</partinfo>
  
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A teichuronopeptide (TUP) is one of major structural components of the cell wall of the facultative alkaliphilic strain Bacillus lentus C-125. A mutant defective in TUP synthesis grows slowly at alkaline pH. It is a highly acidic copolymer composed exclusively of two polymers,one consisting of uronic acids and the other consisting of acidic amino acids. The TUP encoded by gene tupA of strain C-125 is a copolymer of polyglutamic acid (PGlu) and polyglucuronic acid (PGlcU).
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Gene tupA was cloned from the chromosomal DNA of facultative alkaliphilic strain Bacillus lentus C-125. The primary translation product of this gene, TupA, is likely a cytoplasmic protein(57.3 kDa) consisting of 489 amino acid residues.It was demonstrated that TupA is involved in the synthesis of TUP,a copolymer of polyglutamic acid (PGlu) and polyglucuronic acid (PGlcU),which is one of major structural components in the cell wall of the Bacillus lentus C-125 and can neutralize the extracellular hydroxyl. A mutant defective in TUP synthesis grows slowly at alkaline pH,indicating this gene plays a key role in pH homeostasis and increases the alkali resistance of Bacteria.
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Revision as of 07:15, 28 October 2017


C125-TupA

Gene tupA was cloned from the chromosomal DNA of facultative alkaliphilic strain Bacillus lentus C-125. The primary translation product of this gene, TupA, is likely a cytoplasmic protein(57.3 kDa) consisting of 489 amino acid residues.It was demonstrated that TupA is involved in the synthesis of TUP,a copolymer of polyglutamic acid (PGlu) and polyglucuronic acid (PGlcU),which is one of major structural components in the cell wall of the Bacillus lentus C-125 and can neutralize the extracellular hydroxyl. A mutant defective in TUP synthesis grows slowly at alkaline pH,indicating this gene plays a key role in pH homeostasis and increases the alkali resistance of Bacteria.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 969
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 789
    Illegal BsaI.rc site found at 1181