Difference between revisions of "Part:BBa K2324012"

Revision as of 18:12, 1 November 2017

pJ23100_FimOp

This part contains the fim operon under control of a constitutive promoter. When co-transformed with FimH constructs from our project, which have rhamnose-inducible promoters, modified type I pili may be produced. The part requires no induction, and therefore is able to continuously produce the large mass of protein encoded in the operon.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 7
Illegal NheI site found at 30
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 2648
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 931
Illegal AgeI site found at 962
1000
COMPATIBLE WITH RFC[1000]

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K2324012 short</partinfo>
-This part contains the <i> fim operon </i> under control of a constitutive promoter. When co-transformed with the other parts from our project, which have rhamnose-inducible promoters, it will help to synthesise modified type I pilus structures. The part requires no induction, and therefore is able to continuously produce the large mass of protein encoded in the operon.
+This part contains the <i> fim operon </i> under control of a constitutive promoter. When co-transformed with FimH constructs from our project, which have rhamnose-inducible promoters, modified type I pili may be produced. The part requires no induction, and therefore is able to continuously produce the large mass of protein encoded in the operon.
 <!-- Add more about the biology of this part here