Difference between revisions of "Part:BBa K2324006"
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<partinfo>BBa_K2324006 short</partinfo> | <partinfo>BBa_K2324006 short</partinfo> | ||
| − | This part synthesises a FimH adhesin protein fused with | + | This part synthesises a FimH adhesin protein fused with sfGFP at its 225th amino acid residue. The coding sequence |
is under the control of a rhamnose-inducible promoter, with a B0034 RBS and a B0015 terminator. The part, when induced, | is under the control of a rhamnose-inducible promoter, with a B0034 RBS and a B0015 terminator. The part, when induced, | ||
produces a fluorescent FimH protein that should involve itself in pilus biosynthesis when co-transformed with a plasmid | produces a fluorescent FimH protein that should involve itself in pilus biosynthesis when co-transformed with a plasmid | ||
Revision as of 17:34, 30 October 2017
pRha_FimH_225sfGFP
This part synthesises a FimH adhesin protein fused with sfGFP at its 225th amino acid residue. The coding sequence is under the control of a rhamnose-inducible promoter, with a B0034 RBS and a B0015 terminator. The part, when induced, produces a fluorescent FimH protein that should involve itself in pilus biosynthesis when co-transformed with a plasmid containing the fim operon .
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 451
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]