Difference between revisions of "Part:BBa K2259010"
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===Usage and Biology=== | ===Usage and Biology=== | ||
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[[Image:Bielefeld2012_ECOL3LFermentation.jpg|450px|thumb|left|'''Test Figure:''' Fermentation of ''E. coli'' KRXwith <partinfo>BBa_K863005</partinfo> (ECOL) in an Infors Labfors Bioreactor, scale: 3 L, [http://2012.igem.org/Team:Bielefeld-Germany/Protocols/Materials#Autoinduction_medium autoinduction medium] + 60 µg/mL chloramphenicol, 37 °C, pH 7, agitation on cascade to hold pO<sub>2</sub> at 50 %, OD<sub>600</sub> measured every 30 minutes.]] | [[Image:Bielefeld2012_ECOL3LFermentation.jpg|450px|thumb|left|'''Test Figure:''' Fermentation of ''E. coli'' KRXwith <partinfo>BBa_K863005</partinfo> (ECOL) in an Infors Labfors Bioreactor, scale: 3 L, [http://2012.igem.org/Team:Bielefeld-Germany/Protocols/Materials#Autoinduction_medium autoinduction medium] + 60 µg/mL chloramphenicol, 37 °C, pH 7, agitation on cascade to hold pO<sub>2</sub> at 50 %, OD<sub>600</sub> measured every 30 minutes.]] | ||
Revision as of 19:39, 14 October 2017
Rop protein - global copy number inhibitor (SynORI framework)
Rop (also known as repressor of primer) is a small protein responsible for keeping the copy number of ColE1 and related bacterial plasmids low in E. coli. Inhibits plasmid replication of all SynORI framework plasmid groups non-specifically, also other ColE1 replicon plasmids.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]