Difference between revisions of "Part:BBa K2336034:Design"

 
Line 7: Line 7:
  
 
===Design Notes===
 
===Design Notes===
−
None.
 
−
 
  
 +
We amplificated the oprF and FLAG-LBT separately as the amount of gene synthesized by IDT is too little.Then,we linked these two genes and got the plasmid with these two genes.
  
 
===Source===
 
===Source===
  
−
None.
+
Synthesized by IDT.And we fused 2 sequences(opRF and 3*LBT).
 +
 
  
 
===References===
 
===References===

Revision as of 15:13, 31 October 2017


oprF-GS-FLAG-LBT12-GS-LBT12-GS-LBT12


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 884
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 317
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 953
    Illegal AgeI site found at 5
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 121


Design Notes

We amplificated the oprF and FLAG-LBT separately as the amount of gene synthesized by IDT is too little.Then,we linked these two genes and got the plasmid with these two genes.

Source

Synthesized by IDT.And we fused 2 sequences(opRF and 3*LBT).


References