Difference between revisions of "Part:BBa K2336026"
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The report part of our circuit. Oprf can be expressed as an anchor on the cell membrane to make sure the LBT can combine with terbium (Tb3 +) on the surface of the bacteria. | The report part of our circuit. Oprf can be expressed as an anchor on the cell membrane to make sure the LBT can combine with terbium (Tb3 +) on the surface of the bacteria. | ||
| − | < | + | <h1>'''Characterization'''</h1> |
| − | + | ||
| + | This is a part for recycling.LBT1（lanthanide binding tag）can bind the lanthanide ions and it can be expressed at the cell membrane of E.coli with the help of oprF.We set the 'FLAG' between oprF and LBT for fluorescence detection.In our experiment,we let the LBT1 be expressed successfully which means that the lanthanide ions in the water would be bound and recycled effectively after we put the in the water. | ||
| + | [[File:oprF-LBT.jpg|400px|thumb|center|Figure1-1:oprF-LBT]] | ||
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Revision as of 12:35, 30 October 2017
oprF-GS-FLAG-LBT1-GS-LBT1-GS-LBT1
The report part of our circuit. Oprf can be expressed as an anchor on the cell membrane to make sure the LBT can combine with terbium (Tb3 +) on the surface of the bacteria.
Characterization
This is a part for recycling.LBT1（lanthanide binding tag）can bind the lanthanide ions and it can be expressed at the cell membrane of E.coli with the help of oprF.We set the 'FLAG' between oprF and LBT for fluorescence detection.In our experiment,we let the LBT1 be expressed successfully which means that the lanthanide ions in the water would be bound and recycled effectively after we put the in the water.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 72
Illegal NheI site found at 749 - 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 639
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 3
Illegal AgeI site found at 933 - 1000COMPATIBLE WITH RFC[1000]