Difference between revisions of "Part:BBa I739013"
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Stefan Luzi (Talk | contribs) (→Purpose) |
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===Purpose=== | ===Purpose=== | ||
− | <p>This Biobrick was designed for the [http://parts.mit.edu/igem07/index.php/ETHZ ETHZ iGEM 2007 project] and belongs to the constitutive part of the system. | + | <p>This Biobrick was designed for the [http://parts.mit.edu/igem07/index.php/ETHZ ETHZ iGEM 2007 project] and belongs to the constitutive part of the system. This composite is cloned into plasmid [https://parts.igem.org/wiki/index.php/Part:BBa_I739201 pBR322BB1]</p> |
===Testing=== | ===Testing=== |
Revision as of 09:35, 23 October 2007
Composite of BBa_I739001, BBa_I739002 and BBa_I739003
Part Structure
This composite Biobrick consists of part BBa_I739001, part BBa_I739002 and part BBa_I739003.
Purpose
This Biobrick was designed for the [http://parts.mit.edu/igem07/index.php/ETHZ ETHZ iGEM 2007 project] and belongs to the constitutive part of the system. This composite is cloned into plasmid pBR322BB1
Testing
Checked for uniqueness of restriction enzyme cleavage sites:
Eco: ok
Xba: ok
Spe: ok
Pst: ok
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30
Illegal NheI site found at 898
Illegal NheI site found at 921
Illegal NheI site found at 2257
Illegal NheI site found at 2280 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 2099
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]