Difference between revisions of "Part:BBa K2275007"
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− | + | This composite part encodes nitrite reductase cluster (nirB and nirD) which derived from E. coli K-12 MG1655 strain. To achieve our goal of regulation, we choose lacI regulated promoter R0010 and B0034 RBS for high expression level . | |
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Revision as of 16:50, 26 October 2017
PlacI-B0034-NiR cluster
This composite part encodes nitrite reductase cluster (nirB and nirD) which derived from E. coli K-12 MG1655 strain. To achieve our goal of regulation, we choose lacI regulated promoter R0010 and B0034 RBS for high expression level .
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Unknown
- 21INCOMPATIBLE WITH RFC[21]Unknown
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 2093
Illegal AgeI site found at 551
Illegal AgeI site found at 947
Illegal AgeI site found at 2105 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 401