Difference between revisions of "Part:BBa K2368007"
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<partinfo>BBa_K2368007 short</partinfo>
 
<partinfo>BBa_K2368007 short</partinfo>
  
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<p>This part consists of the promoter gal1/gal10, sweetness receptor T1R3 and the terminator CYC1. The promoter gal1/gal10 is a bi-directional galactose-inducible promoter, which requires two percent of galactose to induce twelve hours to elicit protein expression. T1R3 is the sweetness recptor of the human tonge cells belonging to C type G protein coupled receptor.</p>  
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<p>This part consists of the promoter gal1/gal10, sweetness receptor T1R2 and the terminator ADH1. The promoter gal1/gal10 is a bi-directional galactose-inducible promoter, which requires two percent of galactose to induce twelve hours to elicit protein expression. T1R3 is the sweetness recptor of the human tonge cells belonging to C type G protein coupled receptor. </p>  
  
 
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Revision as of 02:48, 14 October 2017


Instruction

PGal 1/10-T1R2-CYC1t

This part consists of the promoter gal1/gal10, sweetness receptor T1R2 and the terminator ADH1. The promoter gal1/gal10 is a bi-directional galactose-inducible promoter, which requires two percent of galactose to induce twelve hours to elicit protein expression. T1R3 is the sweetness recptor of the human tonge cells belonging to C type G protein coupled receptor.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 563
    Illegal BglII site found at 2061
    Illegal BglII site found at 2481
    Illegal BglII site found at 2643
    Illegal BamHI site found at 674
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 291
    Illegal AgeI site found at 1572
    Illegal AgeI site found at 2790
  • 1000
    COMPATIBLE WITH RFC[1000]