Difference between revisions of "Part:BBa K2429012:Design"

 
(→‎Design Notes)
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===Design Notes===
 
===Design Notes===
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human codon optimized
+
This basic part entry vector is flanked by L1 and L2 sites, which are used to denote a gene.
−
 
+
−
 
+
  
 
===Source===
 
===Source===

Revision as of 14:55, 26 October 2017


pENTR Ms2


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 294
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 294
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 294
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 294
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 294
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This basic part entry vector is flanked by L1 and L2 sites, which are used to denote a gene.

Source

This part was obtained from another researcher who was kind enough to give us the sequence and physical plasmid containing the protein with the DDX6 domain sequence attached to the Ms2 sequence.

References